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. Author manuscript; available in PMC: 2011 May 18.
Published in final edited form as: J Immunol. 2011 Feb 4;186(6):3421–3431. doi: 10.4049/jimmunol.1000569

FIGURE 6.

FIGURE 6

IFN-α or SLE patient plasma activates NK cells from healthy controls in vitro. A, Freshly isolated PBMCs from healthy controls were stimulated with IL-2 (100 U/ml) and then IFN-α (1000 U/ml) or IL-2 (100 U/ml) plus IFN-α (1000 U/ml). Cultured cells were harvested at the indicated time points and analyzed. The ratios of CD69+ NK cells (left panel), CD226+CD69+ NK cells (middle panel), and CD226−CD69+ NK cells (right panel) were analyzed by flow cytometry. One representative example from three independent experiments is shown. B, PBMCs from healthy controls were cultured with medium or four types of plasma, including the plasma from healthy controls, from SLE patients in remission, from active SLE patients, and from active SLE patients with IFN-α–neutralizing Ab. For each type of plasma, three independent samples were adopted repeatedly. Cultured cells were harvested and analyzed as in A. Data are shown as mean ± SEM from three independent experiments. Significance was determined using one-way ANOVA, followed by the Bonferroni post hoc test.