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. Author manuscript; available in PMC: 2011 May 18.

Published in final edited form as: Cell Mol Bioeng. 2010 Dec;3(4):376–386. doi: 10.1007/s12195-010-0132-0

Fig. 2 — The ALP activity and calcium deposition examined in rat primary osteoblasts after 6 or 12 h stimulation with increasing ( 5-10-15 dyn/cm²) or decreasing ( 15-10-5 dyn/cm²) FSS relative to static control (=1). The cacium and ALP were normalized with total protein of cells. To indicate significant difference between pair comparison, the following symbals are used: * FSS vs the static control; # stepwise increasing FSS vs decreasing FSS for the same stimulation period (6 or 12h); + 6 h vs 12 h of the same increasing/decreasing FSS pattern.

Inline graphic — The ALP activity and calcium deposition examined in rat primary osteoblasts after 6 or 12 h stimulation with increasing ( 5-10-15 dyn/cm²) or decreasing ( 15-10-5 dyn/cm²) FSS relative to static control (=1). The cacium and ALP were normalized with total protein of cells. To indicate significant difference between pair comparison, the following symbals are used: * FSS vs the static control; # stepwise increasing FSS vs decreasing FSS for the same stimulation period (6 or 12h); + 6 h vs 12 h of the same increasing/decreasing FSS pattern.