Figure 10.

Effects of intrathecal wortmannin or rapamycin on intraplantar carrageenan-induced phosphorylation of 4EBP and S6 ribosomal protein. A, C, Representative Western blots showing p4EBP, total 4EBP, and β-actin (b-actin) (A) and pS6, total S6, and β-actin (C) in the ipsilateral lumbar spinal dorsal quadrant at 1 h after carrageenan. Rats received intrathecal vehicle (3% DMSO, 10 μl) or wortmannin (wort; 4.5 μg) 15 min before carrageenan. E, Western blots showing pS6, total S6, and β-actin in rats received intrathecal vehicle (3% DMSO, 10 μl) or rapamycin (rapa; 3 μg) before carrageenan. B, D, F, Histograms represent the mean p4EBP and pS6 levels with respect to the vehicle group. The data are presented as mean ± SEM of four or five rats per group. *p < 0.05 compared with vehicle by t test.