Skip to main content
. Author manuscript; available in PMC: 2012 Jun 15.
Published in final edited form as: Vet Immunol Immunopathol. 2011 Mar 16;141(3-4):258–266. doi: 10.1016/j.vetimm.2011.03.014

Figure 1.

Figure 1

Representative dot plots of CD4 T cells labeled with anti-CD4, -CD45R0, and -CD25, showing the gating strategy used to electronically isolate activated proliferating cells for analysis, (SSC vs FSC). Panel A, electronic gates were used to identify and color code resting (red) and activated proliferating (blue) cells. Panel B, an additional gate was used to isolate cell subsets for analysis (FSC vs CD4). Panel C, gated CD4 cells showing relative proportion of naïve and CD25- and CD25+ memory CD4 T cells. Panel D, gated CD4 cells showing the proportion of naïve and memory T cells expressing CD25. Panels E and F, additional gates were placed on resting and activated cells to show the relative proportion of resting and activated naïve and memory CD4 cells expressing CD25. Note that all activated CD4 cells expressed CD25. For presentation of the data, the proportion of activated cells present in preparations of PBMC cultured in RPMI alone (as shown in panel C) were subtracted from the proportion of activated cells present in preparations of PBMC cultured in jPPD or SAg.