Table I.
Target gene | Forward (5′ – 3′) | Reverse (5′-3′) | Source |
---|---|---|---|
Nested PCR Primers | |||
IS900(1st set) | GTTCGGGGCCGTCGCTTAGG | GAGGTCGATCGCCCACGTGA | (Chamberlin et al., 2001) |
IS900(2nd set) | ATGTGGTTGCTGTGTTGGATGG | CCGCCGCAATCAACTCCAG | |
RT-PCR Primers | |||
β-actin | GGAATCCTGCGGCATTCAC | GGATGTCGACGTCACACTTCA | This study |
GAPDH | GCATCGTGGAGGGACTTATGA | GGGCCATCCACAGTCTTCTG | (Stabel et al., 2009) |
IFN-γ | CTTGAATGGCAGCTCTGAGAAA | TGCAGATCATCCACCGGAAT | (Sweeney et al., 2006) |
IL17 | GGACTCTCCACCGCAATGAG | TGGCCTCCCAGATCACAGA | This study |
IL22 | GAGGTGCTGTTCCCCCAAT | GAAGGGCACCACCTTTTCC | This study |