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. Author manuscript; available in PMC: 2012 May 10.
Published in final edited form as: Brain Res. 2011 Mar 9;1389:35–49. doi: 10.1016/j.brainres.2011.03.006

Figure 5. Embryonic brain stab injury triggers widespread pro-inflammatory changes.

Figure 5

A. COX-2 and GAPDH mRNA expression in optic tecta from embryos injured unilaterally on E11 and sacrificed on E14 (E11–13) or injured on E15 and sacrificed on E18 (E15–E18) determined by Northern blot analysis. As a sham control (S), tecta from embryos in which eggs were windowed at E11 or E15 and collected in E15 and E18, respectively, were used. A representative Northern blot is shown. B. COX-2 mRNA levels from injured I (ipsilateral) and uninjured C (contralateral) tecta were normalized with respect to GAPDH mRNA levels using Quantity One image analysis software (Bio-Rad). Data represent the average ± the standard deviation from three independent embryos. Tukey HSD test was used after one-way ANOVA to generate the p values. *: p<0.05. C. Expression of GFAP, COX-2, HIF-1 by in situ hybridization in three serially adjoining sections around the stab-wound area (arrow) of embryos injured unilaterally on E15 and sacrificed on E18.