Detection of NFIA (A, B), GLAST (C, D) and SOX9 (E, F) mRNAs in serially adjoining cross-sections of the tectal laminae three days after stab injuries performed at E11. Increased expression levels of astrocyte precursor markers in Ip (ipsilateral) injured tecta (B, D and F) with respect to the Co (contralateral) side (A, C and D) in the VZ (arrows) and scattered throughout the tectal laminae (B arrowheads) suggests activation of astrocyte differentiation. Scale bar: 100μm. Results depicted in this figure were consistently observed in multiple independent embryos (3 embryos).