TABLE 2.
Distribution and transposition sites in lines A and Ba
| Locusb | Chromosome | Chromosome positionc
|
Gene hitd
|
||||
|---|---|---|---|---|---|---|---|
| Ensembl | Celera | Ensembl ID | Celera ID | Product name | Insertion site | ||
| Aa1 | 5 | 133339641 | 128744770 | ENSMUSG00000029675 | mCG16716 | Elastin precursor | Intron |
| Aa2 | 6 | 33263044 | 30087408 | None | mCG1029516 | PD | Intron |
| Aa3 | 7 | 84409604 | 88953421 | None | None | None | None |
| Aa4 | 9 | 124539645 | 120559087 | ENSMUSG00000025786 | mCG117266 | RIKEN cDNA 1810006O10 | Intron |
| ENSMUSG00000025785 | None | RIKEN cDNA 2610002K22 | Upstream | ||||
| Aa5 | 14 | 18064759 | 16317947 | ENSMUSG00000021778 | None | RIKEN cDNA 1700112E06 | Intron |
| Aa6 | 14 | 30096601 | 28201695 | ENSMUSG00000041093 | mCG50091 | Glutamate receptor delta chain | Intron |
| Aa7 | 14 | 30960037 | 29087128 | None | None | None | None |
| Aa8 | 14 | 33434637 | 31519205 | ENSMUSESTG00000017053 | mCG1034632 | RIKEN cDNA 4930581F23 | Intron |
| Aa9*1 | 14 | 34035613 | 32117336 | None | mCG52624 | PD | Intron |
| Aa10*1 | 14 | 34036471 | 32118194 | None | mCG52624 | PD | Intron |
| Aa11*1 | 14 | 34046049 | 32129793 | None | mCG52624 | PD | Intron |
| Aa12*1 | 14 | 34061403 | 32145076 | None | mCG52624 | PD | Intron |
| Aa13*1 | 14 | 34062455 | 32146128 | None | mCG52624 | PD | Intron |
| Aa14*1 | 14 | 34146022 | 32230175 | None | mCG52624 | PD | Intron |
| Aa15*1 | 14 | 34239318 | 32323804 | None | mCG52624 | PD | Intron |
| Aa16*1 | 14 | 34350876 | 32435487 | None | mCG52624 | PD | Intron |
| Aa17 | 14 | 34383025 | 32467640 | None | None | None | None |
| Aa18 | 14 | 34571020 | 32654834 | None | mCG1034432 | PD | Intron |
| Aa19 | 14 | 34720654 | 32806705 | None | None | None | None |
| Aa20 | 14 | 35357392 | 33431825 | None | None | None | None |
| Aa21 | 14 | 36288530 | 34335172 | ENSMUSG00000021795 | mCG10221 | Surfactant associated protein D | Intron |
| Aa22 | 14 | 39282319 | 40628445 | None | None | None | None |
| Aa23 | 14 | 40524943 | 41877684 | ENSMUSESTG00000018516 | None | EST | Intron |
| None | mCG12117 | Otx2 | Upstream | ||||
| Aa24 | 14 | 44414710 | 46537768 | ENSMUSESTG00000022818 | None | EST | Upstream |
| None | mCG18700 | PD | Upstream | ||||
| Aa25 | 14 | 62261532 | 65877739 | None | mCG60014 | Proteoglycan 3 | Intron |
| Aa26 | 14 | 63049150 | 66650853 | None | mCG1037549 | PD | Upstream |
| Aa27 | 14 | 78965759 | 82384631 | None | None | None | None |
| Aa28 | 15 | 42404980 | 39726489 | None | mCG1044549 | PD | Intron |
| Ab1 | 4 | 50509424 | 48625949 | None | None | None | None |
| Ab2 | 8 | 110975125 | 112292150 | ENSMUSESTG00000041903 | mCG61536 | EST | Downstream |
| ENSMUSESTG00000041911 | None | EST | Upstream | ||||
| Ab3 | 10 | 92362613 | 91142273 | ENSMUSESTG00000002022 | None | EST | Intron |
| None | mCG62941 | PD | Upstream | ||||
| Ab4 | 12 | 14320535 | 11576377 | None | None | None | None |
| Ab5*1 | 14 | 34219590 | 32304087 | None | mCG52624 | PD | Intron |
| Ab6 | 14 | 34385669 | 32470284 | None | None | None | None |
| Ab7 | 14 | 35540838 | 33615751 | ENSMUSG00000037842 | mCG49009 | Protein tyrosine phosphatase IVA | Upstream |
| Ab8 | 14 | 115846358 | 121067394 | ENSMUSG00000025551 | None | FGF-14 | Intron |
| None | mCG1025834 | PD | Intron | ||||
| Ba1 | 1 | 73055946 | 69942808 | ENSMUSG00000026187 | mCG121781 | Ku autoantigen | Intron |
| Ba2 | 3 | 12507203 | 9455812 | None | mCG1028528 | PD | Upstream |
| Ba3 | 3 | 13772180 | 10716340 | None | None | None | None |
| Ba4 | 3 | 47252396 | 43663327 | None | mCG1049778 | PD | Intron |
| Ba5 | 3 | 64738743 | 60756385 | ENSMUSG00000027824 | mCG6370 | Putative pheromone receptor V2 | Intron |
| Ba6 | 3 | 76346410 | 72743600 | None | None | None | None |
| Ba7 | 3 | 79419969 | No hit | None | None | None | None |
| Ba8 | 3 | 90606788 | 86803854 | ENSMUSG00000027950 | mCG22243 | Chrnb2 | Upstream |
| ENSMUSG00000042579 | None | RIKEN cDNA 4632404H12 | Downstream | ||||
| Ba9 | 3 | 92937841 | No hit | None | None | None | None |
| Ba10 | 3 | 93338427 | 91452377 | None | mCG1042765 | PD | Upstream |
| Ba11 | 3 | 108208659 | 107046014 | None | None | None | None |
| Ba12 | 3 | 110468799 | 109301041 | ENSMUSG00000027819 | None | Netrin G1 | Intron |
| Ba13 | 3 | 111191473 | 110015146 | None | None | None | None |
| Ba14 | 3 | 124274422 | No hit | None | None | None | None |
| Ba15 | 3 | 125666710 | 128699579 | None | None | None | None |
| Ba16 | 3 | 126228269 | 129185593 | None | None | None | None |
| Ba17 | 3 | 136872386 | 139812954 | None | None | None | None |
| Ba18 | 3 | 139247325 | No hit | ENSMUSG00000028152 | None | Tspan-5 | Intron |
| Ba19 | 3 | 139655523 | 142578508 | None | None | None | None |
| Ba20 | 3 | 139734836 | 142630806 | ENSMUSESTG00000013903 | mCG1045576 | EST | Upstream |
| Ba21 | 3 | 140033976 | 142957610 | None | None | None | None |
| Ba22 | 3 | 140115986 | 143021269 | None | None | None | None |
| Ba23 | 3 | 140177697 | 143087243 | None | None | None | None |
| Ba24 | 3 | 140737961 | 143644800 | None | None | None | None |
| Ba25 | 3 | 141003421 | 143912948 | None | None | None | None |
| Ba26*2 | 3 | 141129016 | 144038837 | None | mCG1045798 | PD | Intron |
| Ba27*3 | 3 | 141226208 | 144135636 | None | mCG1045797 | PD | Intron |
| Ba28*3 | 3 | 141226409 | 144135834 | None | mCG1045797 | PD | Intron |
| Ba29*3 | 3 | 141251735 | 144169231 | None | mCG1045797 | PD | Intron |
| Ba30*4 | 3 | 141442119 | 144355226 | None | mCG1045552 | PD | Intron |
| Ba31*4 | 3 | 141453041 | 144366202 | None | mCG1045552 | PD | Upstream |
| Ba32 | 3 | 141662278 | 144583162 | None | None | None | None |
| Ba33 | 3 | 141687661 | 144608744 | None | None | None | None |
| Ba34 | 3 | 141957604 | 144873099 | None | mCG1045645 | PD | Intron |
| Ba35 | 3 | 142406420 | 145316721 | ENSMUSESTG00000008780 | None | EST | Intron |
| Ba36 | 3 | 142479121 | 145393050 | None | None | None | None |
| Ba37 | 3 | 149178150 | 152125671 | None | mCG1045751 | PD | Intron |
| Ba38 | 3 | 151453999 | No hit | None | None | None | None |
| Ba39 | 3 | 151936034 | 154829309 | None | None | None | None |
| Ba40 | 3 | 154761954 | 157648380 | ENSMUSG00000028202 | None | EST | Intron |
| None | mCG1045569 | PD | Upstream | ||||
| Ba41 | 3 | 159073309 | 161916377 | None | mCG11661 | PD | Upstream |
| Ba42 | 3 | 159422563 | 162257755 | None | mCG1045689 | PD | Intron |
| Ba43 | 4 | 109016854 | 106620571 | ENSMUSESTG00000012197 | None | EST | Downstream |
| None | mCG7419 | PD | Downstream | ||||
| Ba44 | 5 | 30712555 | 26517380 | ENSMUSG00000037511 | None | EST | Intron |
| Ba45 | 5 | 74806690 | 69770958 | None | None | None | None |
| Ba46 | 5 | 97900137 | 94681456 | ENSMUSG00000029324 | None | RIKEN cDNA 1810024J13 | Downstream |
| None | mCG7543 | PD | Upstream | ||||
| Ba47 | 5 | 149049499 | 144802025 | ENSMUSESTG00000018949 | None | EST | Downstream |
| None | mCG1029339 | PD | Intron | ||||
| Ba48 | 7 | 41962607 | 46721472 | None | mCG1033115 | PD | Upstream |
| Ba49 | 8 | 25899555 | 25649727 | ENSMUSG00000031488 | mCG2247 | RIKEN cDNA 4833414G05 | Intron |
| ENSMUSESTG00000035764 | None | EST | Upstream | ||||
| Ba50 | 8 | 66760649 | 66917274 | ENSMUSESTG00000029727 | None | EST | Upstream |
| None | mCG1048987 | PD | Upstream | ||||
| Ba51 | 9 | 53636482 | 47566899 | ENSMUSG00000034584 | mCG129220 | EST | Intron |
| Ba52 | 10 | 101584951 | 100363591 | None | None | None | None |
| Ba53 | 11 | 108198964 | 116126072 | None | mCG54370 | PD | Downstream |
| Ba54 | 13 | 45692264 | No hit | None | None | None | None |
| Ba55 | 14 | 78816293 | 82243983 | None | None | None | None |
| Ba56 | 17 | 37466479 | 38760848 | ENSMUSG00000013094 | None | Olfactory receptor | Upstream |
| None | mCG1034068 | PD | Intron | ||||
| Ba57 | 18 | 40781043 | 38938508 | None | None | None | None |
| Ba58 | 3 | No hit | 72496721 | None | None | None | None |
| Ba59 | 3 | No hit | 132473648 | None | None | None | None |
| Ba60 | 3 | No hit | 139912164 | None | None | None | None |
| Ba61 | 3 | No hit | 144726649 | None | None | None | None |
| Ba62 | 3 | No hit | 146815889 | None | mCG1045783 | PD | Upstream |
| Ba63 | 3 | No hit | 150622778 | None | mCG64937 | PD | Intron |
| Bb1 | 1 | 65738552 | 63658310 | ENSMUSG00000026888 | mCG22301 | Grb14 | Intron |
| Bb2 | 3 | 78123913 | 74530121 | None | None | None | None |
| Bb3 | 3 | 128921395 | 131946395 | None | None | None | None |
| Bb4 | 3 | 134603031 | 137542408 | None | mCG57759 | None | Intron |
| Bb5 | 3 | 136643002 | 139591321 | None | None | None | None |
| Bb6 | 3 | 140069006 | 142985245 | None | None | None | None |
| Bb7 | 3 | 140442067 | 143357645 | None | None | None | None |
| Bb8 | 3 | 140463385 | 143379060 | None | None | None | None |
| Bb9 | 3 | 140721318 | 143627508 | None | mCG53502 | PD | Intron |
| Bb10 | 3 | 140734580 | 143641510 | None | mCG53502 | PD | Downstream |
| Bb11 | 3 | 140959824 | 143863788 | None | None | None | None |
| Bb12*2 | 3 | 141123243 | 144033139 | None | mCG1045798 | PD | Intron |
| Bb13*2 | 3 | 141140455 | 144050261 | None | mCG1045798 | PD | Upstream |
| Bb14*2 | 3 | 141140455 | 144050261 | None | mCG1045798 | PD | Upstream |
| Bb15 | 3 | 141153125 | 144063917 | None | None | None | None |
| Bb16 | 3 | 141153125 | 144063917 | None | None | None | None |
| Bb17 | 3 | 141161352 | 144072145 | None | None | None | None |
| Bb18*3 | 3 | 141216021 | 144124766 | None | mCG1045797 | PD | Intron |
| Bb19*4 | 3 | 141370931 | 144282066 | None | mCG1045552 | PD | Intron |
| Bb20 | 3 | 141533210 | 144447454 | None | None | None | None |
| Bb21 | 3 | 141550152 | 144468179 | None | None | None | None |
| Bb22 | 3 | 141791266 | 144708994 | None | None | None | None |
| Bb23 | 3 | 159306381 | 162143341 | None | None | None | None |
| Bb24 | 9 | 52064672 | 46001369 | None | None | None | None |
| Bb25 | 10 | 52847168 | 50527975 | None | mCG1028869 | None | Upstream |
| Bb26 | 11 | 111938647 | 119846977 | None | None | None | None |
| Bb27 | 13 | 112145008 | 115874575 | None | None | None | None |
| Bb28 | 3 | No hit | 56662465 | None | None | None | None |
| Bb29 | 3 | No hit | 142724083 | None | mCG1045576 | PD | Intron |
a
Sequences flanking transposon insertion sites were searched with the mouse genome databases at both Ensembl and Celera Genomics to determine the chromosome positions of the insertion sites and to identify any insertion events nearby or in the genes. Transposition sites that were aligned with the array of transposon sequences located at the DST or that could not be mapped owing to the repetitive nature of the sequences are not presented. In cases where insertion sites are mapped to two genes, both genes are presented. Eleven insertion sites were determined from testis DNA, and remaining 128 sites were from sperm DNA.
b
The first two letters of the locus name correspond to the name of the parental seed mouse (shown in Fig. 1B and 4D) from which each insertion site was determined. The names of the loci that were mapped to the same gene are indicated by *1 to *4, each representing insertion into the same gene.
c
No hit indicates that a transposition site could not be determined.
d
ID, identification number; PD, gene predicted according to the Celera Genomics database. Based on Celera database parameters, 10 kb upstream and downstream of the transcription units is associated with the same gene identification number. Therefore, insertion sites occuring in these regions are defined as upstream and downstream insertion sites, respectively.