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. Author manuscript; available in PMC: 2012 Jun 10.
Published in final edited form as: Exp Cell Res. 2011 Apr 5;317(10):1368–1381. doi: 10.1016/j.yexcr.2011.03.019

Fig. 6.

Fig. 6

Suppressed CTGF expression contributes to the resistance of HCT-116 cells to the growth inhibitory effects of butyrate. HCT-116 cells (106) were nucleofected with 175 pmol of control (non-specific) or human CTGF siRNA (Santa Cruz Biotechnology) and incubated in two wells of a 6-well plate. At six hours post-transfection the cells were mock-treated or exposed to 5 mM sodium butyrate for 17 hours. The inset is a representative Western blot analysis of the CTGF protein levels in control and CTGF siRNA-nucleofected cells exposed to 5 mM butyrate for 17h. Clonal growth assays were performed as described in Materials and Methods. The clonal growth of butyrate-treated cells is expressed as a percentage of the clonal growth of the mock-treated cells. The clonal growth assays were repeated at least three to six times. Statistically significant differences are marked with asterisks. Bars, SDs.