TABLE 1.
Yeast strains and plasmids used
| Strain or plasmid | Genotype and comments | Reference or source |
|---|---|---|
| Strains | ||
| NOY408-1af | MATaade2-1 ura3-1 his3-11 trp1-1 leu2-3,112 rpa135::LEU2 can1-100 fob1::HIS3 pNOY102 pNOY117 | 27 |
| NOY408-1b | MATaade2-1 ura3-1 his3-11 trp1-1 leu2-3,112 can1-100 | 39 |
| NOY408-1bf | Same as NOY408-1b except fob1::HIS3 | 27 |
| TAK899 | Same as NOY408-1bf except rDNA::URA3 | |
| YK9 | MATaura3-52 trp1-289 leu2-3-112 prb pep4::TRP1 | 33 |
| Plasmids | ||
| pNOY102 | Multicopy plasmid vector; URA3 GAL7-35S rDNA 2μm | 39 |
| pNOY117 | CEN6 ARSH4 TRP1 RPA135 | Nomura lab |
| pEG(KT) | Multicopy plasmid vector; URA3 Leu2-d GAL1-GST 2μm | 38 |
| pTAK900 | pEG(KT) carrying FOB1 | |
| pESC-LEU | Multicopy plasmid vector; LEU2 GAL1-FLAG 2μm | Stratagene |
| pTAK901 | pESC-LEU carrying FOB1 | |
| pTAK991 | Same as pTAK901 except the ORF of FOB1 has a point mutation (H159A) | |
| pTAK992 | Same as pTAK901 except the ORF of FOB1 has a point mutation (H164A) | |
| pTAK993 | Same as pTAK901 except the ORF of FOB1 has a point mutation (C193A) | |
| pTAK994 | Same as pTAK901 except the ORF of FOB1 has a point mutation (C196A) | |
| pTAK995 | Same as pTAK901 except the ORF of FOB1 has a point mutation (D291A) | |
| YEp24 | Multicopy plasmid vector; URA3 2μm pBR322 Ampr Tcr | New England Biolabs |
| pTAK902.1 to -9 | YEp24 carrying various fragments (1 to 9 in Fig. 3B) around the RFB | |
| pTAKc1 to -f4 | YEp24 carrying subfragments (24 bp, c1 to f4 in Fig. 5C) of the RFB | |
| Yeplac195 | Multicopy plasmid vector; URA3 2μm | 12 |
| Yep-FOB1 | Yeplac195 carrying FOB1 | 27 |