Figure 5.

Expression of Sema3s in the mPFC. A, RT-PCR showing the expression of sema3A, 3B, 3C, 3D, 3E, 3F, and 3G in mouse head and mPFC at E14.5. Only sema3A, 3C, and 3F can be found in the mPFC (arrows). Splice forms of sema3F are indicated by arrowheads (Kusy et al., 2003). Actin serves as a control. B–G, In situ hybridization on coronal sections shows sema3C and sema3F expression in the E14.5 (B, E) and E18.5 (C–F) mPFC. Sema3C is expressed within the SVZ and IZ and weakly within the upper layers of the CP, whereas sema3F is almost exclusively expressed in the CP.