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. Author manuscript; available in PMC: 2012 Jun 11.
Published in final edited form as: Eur J Pharmacol. 2011 Jan 3;660(1):181–187. doi: 10.1016/j.ejphar.2010.10.114

Fig. 4.

Fig. 4

Colocalization of estrogen receptor α (ESR1) and POMC in the adult hypothalamus. (A) Immunofluorescence of the rat arcuate nucleus against POMC-derived ACTH (green), ESR1 (red) and the superimposition of both images (merge). Colocalization, denoted by arrowheads, is seen between nuclear ESR1 and cytosolic ACTH. (B) Immunofluorescence against ESR1 (red) on a coronal hypothalamic section of a Pomc-EGFP transgenic mouse (green). 3v: third ventricle. (C) Higher magnification of the same experiment as on B. Colocalization of ESR1 and EGFP is indicated in several neurons with white arrowheads.