Table 1.
Primer sequence used in cDNA synthesis and ACP™-based PCR
| Use | Primer name | Sequence |
|---|---|---|
| cDNA synthesis primer | dT-ACP1 | 5'-CTGTGAATGCTGCGACTACGATIIIII (T) 18-3' |
| Reverse primer | dT-ACP2 | 5'-CTGTGAATGCTGCGACTACGATIIIII (T) 15-3' |
| Arbitrary primer | ACP1 | 5'-GTCTACCAGGCATTCGCTTCATIIIIIGCCATCGACC-3' |
| (Forward primer) | ACP2 | 5'-GTCTACCAGGCATTCGCTTCATIIIIIAGGCGATGCC-3' |
| ACP3 | 5'-GTCTACCAGGCATTCGCTTCATIIIIICCGGAGGATG-3' | |
| ACP4 | 5'-GTCTACCAGGCATTCGCTTCATIIIIIGCTGCTCGCG-3' | |
| ACP5 | 5'-GTCTACCAGGCATTCGCTTCATIIIIIAGTGCGCTCG-3' | |
| ACP6 | 5'-GTCTACCAGGCATTCGCTTCATIIIIIGGCCACATCG-3' | |
| ACP7 | 5'-GTCTACCAGGCATTCGCTTCATIIIIICTGCGGATCG-3' | |
| ACP8 | 5'-GTCTACCAGGCATTCGCTTCATIIIIIGGTCACGGAG-3' | |
| ACP9 | 5'-GTCTACCAGGCATTCGCTTCATIIIIIGATGCCGCTG-3' | |
| ACP10 | 5'-GTCTACCAGGCATTCGCTTCATIIIIITGGTCGTGCC-3' | |
| ACP11 | 5'-GTCTACCAGGCATTCGCTTCATIIIIICTGCAGGACC-3' | |
| ACP12 | 5'-GTCTACCAGGCATTCGCTTCATIIIIIACCGTGGACG-3' | |
| ACP13 | 5'-GTCTACCAGGCATTCGCTTCATIIIIIGCTTCACCGC-3' | |
| ACP14 | 5'-GTCTACCAGGCATTCGCTTCATIIIIIGCAAGTCGGC-3' | |
| ACP15 | 5'-GTCTACCAGGCATTCGCTTCATIIIIICCACCGTGTG-3' | |
| ACP16 | 5'-GTCTACCAGGCATTCGCTTCATIIIIIGTCGACGGTG-3' | |
| ACP17 | 5'-GTCTACCAGGCATTCGCTTCATIIIIICAAGCCCACG-3' | |
| ACP18 | 5'-GTCTACCAGGCATTCGCTTCATIIIIICGGAGCATCC-3' | |
| ACP19 | 5'-GTCTACCAGGCATTCGCTTCATIIIIITCTGCGAGC-3' | |
| ACP20 | 5'-GTCTACCAGGCATTCGCTTCATIIIIIGACGTTGGCG-3' |
ACP, annealing control primer; the polydeoxyinosine [poly (dI)] linkers are underlined. In the table, "I" represents deoxyinosine