Figure 5. Culture B16 and D122 cells uptake exogenously added α-Syn.

Cells were seeded on cover slips in 12 wells plates one day before the addition of purified α-Syn (1 µM) into standard serum-containing medium. Control sister cultures were grown and maintained in parallel but without the addition of recombinant α-Syn. Cells were then stained for the presence of α-Syn using anti human α-Syn antibody, LB509 (red signal).