Figure 6. α-Syn expression in B16 and E0771 enhances cell proliferation.

(a). Stable poly-clones of B16 cells over expressing either human wt α-Syn, human β-Syn, amyloid precursor protein carrying the Swedish mutation (APPsw) or mock-transfected, were seeded in a 96-well plates at 5×10³ cells per well. Proliferation was determined by the fluorescence ratio at 560ex/590em and normalized to the mock-transfected cells. A representative result of cells 48 hours post seeding. Mean ± SE of n = 6 wells out of three repeats. (b) Stable poly-clones of E0771 and (c) D122, seeded and measured as in (a). *, p<0.05, Mann-Whitney test.