Table 2. IC₅₀ values^a for inhibition of ATP hydrolysis by YscN optimized catalytic domain for selected small molecules.

Compound ID	IC50, µM
7812	41 b
7146	16.5±1.5
1504	18.2±4.9
7086	32 a
9652	50 a
7832	70 a

The ATPase inhibition was measured by following ADP release at 37°C as described under Materials and Methods.

^aThe value was determined from the best fit as the concentration at which 50% inhibition of ATPase activity was observed.