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. 2001 Mar;12(3):675–684. doi: 10.1091/mbc.12.3.675

Figure 2.

Figure 2

Down-regulation of chimeric mutant T200V type I receptors. Cell surface binding in response to 10 ng/ml GM-CSF was performed as described in MATERIALS AND METHODS. Each point represents the mean (± SE) for all clones of the series assayed in duplicate from two independent experiments. (A) AKR-2B fibroblast clones T200V-10 and -12 (○) are compared with A105 wild-type control (●). (B) Mv1Lu epithelial clones T200V-2 and -7 (□) are compared with the mean of MB-9 and -18 as wild-type control (▪). (C) Mean percentage of down-regulation of two different epithelial (▪, □) and three fibroblast (●, ○) cell lines. Stable clones expressing the T200V mutation (open symbols) in Mv1Lu (clones 2 and 7) and MDCK (clones 13, 17, 32, and 38) epithelial lines and 3T3-NIH (clones 6, 7, and 9), AKR-2B (clones 10 and 12), and NRK-49F (clones 1 and 3) fibroblast lines were used. Control wild-type expressing clones (closed symbols) used for comparison were Mv1Lu-9 and -18, MDCK-1 and -5, NIH-3T3-2 and -10, AKR-2B clone A105, and NRK-49F-7.