Figure 1. Age-dependent survival and functional decline in a typical JK1107 (glp-1) C. elegans cohort.

Nematodes are maintained at the restrictive temperature of 25.5°C. (A) Survival curve. Mean lifespan 12.3 days (n = 134). (B) Functional decline as judged by reduction in pharyngeal pumping rate (mean ± SEM) with age. Worms exhibiting irregular or sporadic pumping were excluded. (C) Motility phenotype of surviving worms at different ages. Scoring was carried out according to [40]. Briefly, class “A” worms move vigorously, leaving sinusoidal tracks. Class “B” worms move only in response to touch or appear uncoordinated in locomotion. Class “C” worms cannot move their body but exhibit head and/or tail movements in response to touch.