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. 2011 May 18;6(5):e19444. doi: 10.1371/journal.pone.0019444

Figure 6. MnSOD mutants do not suffer from elevated oxidative damage.

Figure 6

Comparison of ROS related parameters between MnSOD double mutant strain GA480 and WT N2 C. elegans. (A) Averaged fluorescence signal from whole worm DCF-DA generalised ROS assay (n = 7 repeats) of day 4 WT and GA480 animals. Despite lacking both forms of mitochondrial SOD, GA480 animals produce less ROS associated fluorescence signal than WT animals. (B) Analysis of the data from (A); GA480 produce statistically significantly less ROS as measured by our DCF-DA assay (P = 0.024, Student's t-test). (C) Oxidative mtDNA lesions in mtDNA samples (n = 4, each) of day 4 GA480 and WT worms. GA480 does not suffer from significantly elevated oxidative damage as judged by our qRT-PCR assay for oxidative mtDNA damage (P = 0.43, Student's t-test). (D) Oxygen consumption in GA480 vs. WT animals on day 4 of life. The median oxygen consumption was not significantly different between GA480 and WT (P = 0.31, Mann-Whitney test).