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. 2011 May 18;6(5):e19899. doi: 10.1371/journal.pone.0019899

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Zhang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) A paraffin-embedded thin section of a whole pupa showing the overall structure and corresponding regions where chitinases were detected in immunohistochemical analysis as shown in Panels B, C and D. B) Chitinase detected in the abdominal tip and the tail paddles of a pupa by anti-Manduca sexta chitinase 5 polyclonal antibodies (anti-MsCht5) as shown by green color. C) Chitinase detected in certain parts of thorax and developing legs of a pupa by anti-MsCht5 as shown by green color. D) Chitinase detected in the ommatidia of a compound eye by anti-sand fly (Lutzomyia longipalpis) chitinase 8 (anti-sand fly Cht8) polyclonal antibodies as shown by green color.