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. 2011 May 18;6(5):e19912. doi: 10.1371/journal.pone.0019912

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Hansen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The GC-content is depicted in the central part of the map. Open reading frames are shown as colour coded arrows, indicating their function and transcribed direction. The ORFs are divided into the following modules: gem (gene expression modulation), tra (conjugal transfer), mob1 (mobilization 1), rep/stb (replication initiation/toxin-antitoxin plasmid addiction), mob2 (mobilization 2), par (plasmid partitioning) and res (resistance marker). Unique restriction sites are shown at the module junctions. MCS represents the restriction sites PmeI, AbsI, MreI, KpnI, SwaI, NotI and SgrDI.