Fig. 2.

BOCT1 and BOCT2 expression in choroid plexus and cell lines. Total RNA was isolated from the indicated tissues and cell lines and a northern blot was prepared. Probes were prepared for BOCT1 (a) and BOCT2 (b) by random primer labeling using PCR fragments described in methods for library screening. Blots were hybridized overnight at 45°C in Hybrisol and washed at a stringency of 0.2× SSC, 0.5% SDS at 55°C. Hybridized bands were detected and the data captured by phosphoimaging