Table 1.
Primer | Sequence |
---|---|
B0CT1 primers | |
445 (rat) | 5′-AGTGAAGCGGCAGATTGAG-3′ |
446 (rat) | 5′-CAGCTTGGGAGGAGAAGAG-3′ |
B0CT2 primers | |
469 (rat) | 5′-ATCCCGGCGCTGTTCATC-3′ |
470 (rat) | 5′-CGGCTAGC C CA GGCAT GAGG-3′ |
476 (rat, 3′-RACE) | 5′-GGCGGCGGCTACCAGAAGACCCTG-3′ |
477 (rat, 5′-RACE) | 5′-GGGACAGATGATGAGGGCCTGCAA-3′ |
482 (rat, 3′-RACE) | 5′-TGAGGCCAGGGCAGTGAGGATCATG-3′ |
489 (rat) | 5′-AGCGCCCGAAGT CCGTGGAG-3′ |
490 (rat) | 5′-CGCG GCC GGAGA GAATG CTC-3′ |
Primers were designed for BOCT1 and BOCT2 for RT-PCR analysis and for RACE PCR cloning of BOCT2