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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Nat Chem Biol. 2011 Apr 17;7(6):351–358. doi: 10.1038/nchembio.558

Fig. 2. Temperature effects on CRAC channel-dependent gene expression.

Fig. 2

(a) Schematic illustration of NFAT-responsive gene expression system. (b) Protocol used for assaying temperature effects on NFAT-responsive or AP1-responsive luciferase expression. (c) Representative experiment showing luciferase activity of Jurkat-NFAT-Luc cells with the indicated treatment conditions. (d) Fold changes of NFAT-luciferase activity before (left panel) or after (right panel) subtracting Synta1-insensitive portion of the responses [averaged from five separate experiments represented in (c) and normalized to the responses of 37°C-treated cells.]. (e) Representative experiment showing concentration-dependent responses of ionomycin-induced NFAT-luciferase activity under the indicated treatment conditions and fitted with Hill equations. (f) Average fold changes of 100 nM ionomycin/1 µM PMA-induced NFAT-luciferase activity under the indicated conditions (averaged from six separate experiments). (g) Concentration-dependent responses of PMA-induced AP1-dependent luciferase activity in HEK293T cells transfected with the AP1-Luc reporter fitted with Hill equations. (data shown as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001)