Fig. 4. The STIM1 K-domain is essential for heat-induced STIM1 clustering.
(a) CPA-induced store depletion and SOCE (left panel) or temperature responses (right panel) of HeLa cells transfected with the indicated constructs. (b–c) Confocal analysis of HeLa cells co-transfected with Orai1 and the indicated constructs at 25°C (upper panels) or after heating to the indicated temperatures (lower panels). GFP intensity plots represent localization of each construct across the cell as indicated by the white line.