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Dissociation of G proteins from caveolae. Isolated caveolae (V) (20 μg) were diluted in MBS and gently mixed at 4°C for 4 h followed by centrifugation to separate the repelleted caveolar membranes (R) from the soluble phase containing any dissociated proteins (D). An equal volume of starting caveolae (lane SM), R, and D were subjected to Western analysis using antibodies to the indicated proteins.
