Figure 4.

Immunofluorescence microscopy of lipid rafts for colocalization with G proteins and caveolin. MA104 cells (panels A-G) were grown on coverslips, fixed, and incubated with monoclonal antibodies to either folate receptor (panels A, C, E, green labeling) or caveolin (panels B, D, F; green labeling), and polyclonal antibodies to G_q (panels A and B, red/orange), G_i (panels C and D, red/orange), G_s (panel E and F, red/orange), and caveolin (G, red/orange). (Panel H) BLMVEC labeled with 5′NT antibodies (green) and caveolin antibodies (red/orange). Images were overlaid, and the signal overlap is indicated in yellow. Inset: higher magnification of a representative area of the image showing overlapping signals. Magnification of insets, 3×.