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Coimmunoprecipitation of Gq with soluble caveolin. Cytosols were prepared from rat lung homogenates and subjected to immunoprecipitation (IP) with magnetic beads coated with caveolin-1 or G protein antibodies as indicated. Equal volumes of the material bound to the beads was then subjected to Western analysis (WB), using antibodies to caveolin-1 or the indicated G proteins. HC, immunoglobulin heavy chain; LC, immunoglobulin light chain.
