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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Nat Chem Biol. 2011 May 8;7(6):367–374. doi: 10.1038/nchembio.561

Figure 5.

Figure 5

DNA mismatches impede the degradation activity of λ exo in a synergistic manner. (a) Schematics of DNA constructs with periodically sparse (5Ms) and consecutive (2M to 5M) mismatches. (b) Histogram of degradation time obtained from periodically mismatched DNA (5Ms). (c) Representative single molecule FRET time traces taken from DNA constructs with various mismatch configurations outlined in (a). (d) Average degradation time as a function of the number of consecutive mismatches shows that the effective mismatches is non-additive if they are concatenated.