Table 1.

Voxel size, number of slices, and optical planes, and approximate time required to perform stitching, alignment, and segmentation in each reconstruction described in this study.

	Voxel size (μm)	No. of slices planes	No. of optical stitching (min)	Time for alignment (min)	Time for segmentation (min)	Time for
Rough Rec (10×)	1.4 × 1.4 × 40	25	25 (4 fields)	15	20	45
20x Rec	0.7 × 0.7 × 4.5	9	79 (4 fields)	60	45	300
100x Rec	0.14 × 0.14 × 1.6	9	229 (2 fields)	45	45	45
100x Rec individual cells	0.14 × 0.14 × 1.6	9	229 (2 fields)	45	45	240