Frozen sections from E14.5 Chd7Gt/+ embryos demonstrate β-galactosidase (X-gal staining) in CHARGE-related organs including (A) olfactory bulbs (ob), (B) olfactory epithelium (oe), (C) limb, (D) heart (hrt), (E) kidney (kd), (F) enteric neurons of the gut (en), (G) crista (ca) and cochlea (coc) of the inner ear and (H) lens (le) and retina (re) of the eye. All sections are in the coronal orientation.