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Genetic analysis of the role of Yak1p in regulating pseudohyphal growth. Wild-type diploid yeast (MLY61 a/α) or yeast disrupted for YAK1 (DSY1 a/α) were transformed with a control vector (A), or with vectors expressing RAS2VAL19 (B), STE11-4 (C), STE12 (D), TEC1 (E), TPK2 (F), PHD1 (G), or the C terminus of E1A (H). Transformed cells were then transferred to SLAD plates, allowed to grow for 2 d at 30°C, and photographed.
