Figure 7.

Interaction of yeast Yak1p with the E1A C-terminal deletion mutants in the yeast two-hybrid assay. Yeast strain L40 was transformed with expression vectors for the indicated LexA-E1A fusions and expression vectors for YAK1 or CtBP fused to a transcriptional activation domain. Transformed yeast were streaked on nonselective plates (+histidine) and selection plates (−histidine) and allowed to grow at 30°C for 3 d. For each E1A mutant used as “bait,” the indicated numbers are inclusive and refer to the amino acid residues deleted with respect to the 289R E1A protein.