Figure 8.

Coprecipitation of E1A with mammalian homologs of Yak1p. Purified recombinant rat GST-Dyrk1A or human GST-Dyrk1B was incubated with yeast extracts containing either the 243R or 289R E1A proteins (see MATERIALS AND METHODS). The GST-Dyrk protein complexes were then pulled down with glutathione-Sepharose beads and the proteins were analyzed by SDS-PAGE and imunoblotted with a monoclonal antibody against E1A. The empty GST vector was used as a negative control, and the input levels of E1A were as shown.