Figure 9.

Effect of E1A on kinase activity of recombinant rat Dyrk1A. (A) Effect of E1A on autophosphorylation. GST-Dyrk1A was incubated with GST-E1A 243R in phosphorylation buffer for 2 h on ice. Following the addition of [γ-³²P]ATP, samples were incubated for 30 min at 30°C, resolved on 15% SDS-PAGE, and analyzed using a Molecular Dynamics phosphoImager. Lane 1 contains 12 μg of GST-Dyrk1A. Lanes 2–6 contain 12 μg of GST-Dyrk1A with 2, 4, 6, 8, or 12 μg of GST-E1A 243R. Lane 7 contains 12 μg of GST-E1A 243R. (B) Effect of E1A on phosphorylation of histone H3. The assay was performed as described in A except for the presence of histone H3. Fold changes in kinase activity are indicated below each lane.