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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Mol Microbiol. 2011 Mar 7;80(2):319–334. doi: 10.1111/j.1365-2958.2011.07593.x

Figure 2. H2O2 does not eliminate the tyrosyl radical of NrdB.

Figure 2

The expression of nrdB was induced in aerobic LB medium for two hours in wild-type (JEM914) or Hpx− (JEM915) strains harboring pBAD-N-S-nrdB. Cells were washed and concentrated, and where indicated H2O2 was added for 5 min at 37°C. The intact cells were then transferred to EPR tubes and analyzed. HU: hydroxyurea was added to quench the NrdB tyrosyl radical.