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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Mol Microbiol. 2011 Mar 7;80(2):319–334. doi: 10.1111/j.1365-2958.2011.07593.x

Figure 4. Fur and IscR both regulate the expression of nrdHIEF.

Figure 4

Cells bearing a nrdH’-‘lacZ translational fusion were grown in aerobic media. Data represent the mean of three independent cultures. (A) JEM648 (wild type) and JEM657 (Δfur) were grown in glucose/amino acids medium with or without 100 μM of the iron chelator DFO. (B) JEM648 (wild type), JEM657 (Δfur), JEM707 (ΔtonB) and JEM709 (ΔtonB Δfur) were grown in LB medium. (C) Expression of a nrdH’-lac+ transcriptional fusion in LB medium from JEM646 (wild type), JEM762 (ΔiscR), and JEM776 (iscR-C92A/C98A/C104A).