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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Mol Microbiol. 2011 Mar 7;80(2):319–334. doi: 10.1111/j.1365-2958.2011.07593.x

Figure 7. EPR spectra of NrdF expressed in iron-deficient and iron-replete cells.

Figure 7

(A) Iron-deficient (ΔtonB ΔfeoABC ΔzupT) cells with (JEM1121, black) or without (JEM865, red) plasmid pBAD-N-S-2-nrdF. Cells were cultured in aerobic MOPS glucose/amino acids medium, and arabinose was added for 2 hours before harvesting and whole-cell EPR analysis. (B) Iron-replete wild-type cells harboring pBAD-N-S-2-nrdF (JEM1033) were cultured in aerobic LB medium, and arabinose was added for two hours before harvesting and whole-cell EPR analysis (black). Red: 160 mM hydroxyurea was added for 1 hour after nrdF expression prior to EPR analysis.