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. 2011 Feb 8;4(3):335–346. doi: 10.1242/dmm.006411

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© 2011. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 1. — Ethanol-reared flies show reduced viability and developmental delay. (A) The percentage of flies undergoing eclosion (mean + s.e.m.) differs between control (0% ethanol) and ethanol-reared flies (5, 10 and 12% ethanol-food) (Dunnett’s, n=10, *P<0.0001). (B) Duration of development (from egg to adult) is prolonged by ethanol-rearing conditions. Cumulative eclosion rates (percentage of flies undergoing eclosion) of ethanol-reared flies differ from that of control flies (repeated measures ANOVA, n=10, *P<0.0001 between and within groups). Time to 50% total eclosion (at which 50% of flies had eclosed) differs between ethanol-reared and control flies and is indicated with arrowheads (Dunnett’s, n=10, *P<0.0001). Colors correspond to treatment groups shown in A. (C) At eclosion, adult mass is reduced by developmental ethanol exposure (Kruskal-Wallis tests, n=6, *P=0.0032 and 0.0242 for adult females and males, respectively).