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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Cancer Res. 2010 May 11;70(11):4375–4384. doi: 10.1158/0008-5472.CAN-09-4403

Figure 3. ITGA7 inhibits MCM7-chromatin association.

Figure 3

(A) ITGA7 decreases MCM7 association with chromatin. PITT1 or PITT2 cells were treated with or without tetracycline. Chromatin was then purified, electrophoresed on 8% SDS-PAGE and immunoblotted with antibodies against MCM7. Antibodies against Histone 3 and GAPDH were used to examine the purity of the chromatin fraction. Chr-chromatin; NC-non-chromatin. (B) Knocking down of ILK reverse ITGA7 effect on MCM7-chromatin association. PITT1 cells were transfected with siRNA specific for ILK or scramble control, and treated with or without tetracycline. Chromatin association analyses of MCM7 were performed as in (A). (C) Over-expression of ILK exaggerates the inhibition of MCM-chromatin association. PITT1 cells were transfected with pCMV-ILK or pCMVscript, and treated with or without tetracycline. Chromatin association analyses of MCM7 were performed as in (A).