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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Cancer Res. 2010 May 11;70(11):4375–4384. doi: 10.1158/0008-5472.CAN-09-4403

Figure 5. Tumor suppressor activity of ITGA7 is dependent on ILK/MCM7 interaction.

Figure 5

(A) MCM7 mutant that does not bind ILK fails to dissociate from chromatin upon ITGA7 stimulation. MCM7 mutant that contains 58 amino acid ILK binding motif deletion in its N-terminus was transfected into PITT1 cells. Two colonies (#4 and #8) were selected for analysis. Chromatin association analyses were subsequently performed to evaluate its chromatin binding activity. (B) BrdU Labeling and cell cycle analyses of wild type or mutant MCM7 in PITT1 cells. Blue represents G0/G1 phase, purple S phse, and yellow G2/M phase. (C) Colony formation analysis of wild type and mutant MCM7 expressing PITT1 cells.