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. Author manuscript; available in PMC: 2012 Jun 1.

Published in final edited form as: Curr Opin Colloid Interface Sci. 2011 Jun 1;16(3):203–214. doi: 10.1016/j.cocis.2010.12.004

(a) Comparison of dye release from unilamellar vesicles and vesosomes of DPPC on exposure to varying amounts of porcine pancreas phospholipase A₂ at 37° C. PLA₂ induces rapid release from unilamellar DPPC vesicles in a concentration dependent manner (closed symbols), causing complete dye release within 4– 8 hours. Essentially no release beyond background is induced by PLA₂ from vesosomes (open symbols) suggesting that the PLA₂ cannot reach the interior compartments of the vesosome where the dye is sequestered. Adapted and reproduced with permission from ACS from Figure 2 of Boyer et al. ACS Nano. 2007;1:176–82 (2). (b) Dye release from the DPPC within DPPC vesosome has a distinct delay (about 10 hours) during which almost nothing is released. Even in 98% serum, there is only 30% release in 50 hours. Unilamellar DPPC vesicles are completely empty after 2 hours exposure to serum. The exterior membrane protects the interior compartments from the degradation due to the various components of serum such as the HDL and lipases. Adapted and reproduced with permission from ACS from Figure 5 of Boyer et al. ACS Nano. 2007;1:176–82 (2).

(a) Comparison of dye release from unilamellar vesicles and vesosomes of DPPC on exposure to varying amounts of porcine pancreas phospholipase A₂ at 37° C. PLA₂ induces rapid release from unilamellar DPPC vesicles in a concentration dependent manner (closed symbols), causing complete dye release within 4– 8 hours. Essentially no release beyond background is induced by PLA₂ from vesosomes (open symbols) suggesting that the PLA₂ cannot reach the interior compartments of the vesosome where the dye is sequestered. Adapted and reproduced with permission from ACS from Figure 2 of Boyer et al. ACS Nano. 2007;1:176–82 (2). (b) Dye release from the DPPC within DPPC vesosome has a distinct delay (about 10 hours) during which almost nothing is released. Even in 98% serum, there is only 30% release in 50 hours. Unilamellar DPPC vesicles are completely empty after 2 hours exposure to serum. The exterior membrane protects the interior compartments from the degradation due to the various components of serum such as the HDL and lipases. Adapted and reproduced with permission from ACS from Figure 5 of Boyer et al. ACS Nano. 2007;1:176–82 (2).