Table 1.
HPV PCR Primers
| GenBank Accession # | Start to end (bp) | Forward & Reverse (5′ to 3′) | PCR Length | Annealing Temp | Regions |
|---|---|---|---|---|---|
| AF335604.1: HPV6-137 | 1080 to 1217 | F: ACATGCGTCATGTGGAAGAG | 137bp | 52°C | L1 |
| R: AGGCGATAACCCAAAGTTCC | |||||
| M14119.1: HPV 11-182 | 578 to 760 | F: CCTGCAGCCTCCTGACCCTGT | 182bp | 60°C | E7 |
| R: CTCCGTCTGTGCACTCCACAA | |||||
| M14119.1: HPV 11-230 | 291 to 521 | F: TGCAGCGTGTGCCTGTTGCTT | 230bp | 60°C | E6 |
| R: AGCAACGACCCTTCCACTGGT | |||||
| NC001526.1: HPV 16-101 | 497 to 597 | F: TGGACCGGTCGATGTATGT | 101bp | 54°C | E6 & E7 |
| R: CATATATTCATGCAATGTAGGTGTA | |||||
| NC001526.1: HPV 16-173 | 425 to 597 | F: AAGCCACTGTGTCCTGAAGAA | 173bp | 54°C | E6 & E7 |
| R: CATATATTCATGCAATGTAGGTGTA | |||||
| NC001526.1: HPV 16-258 | 382 to 640 | F: AATACAACAAACCGTTGTGTGATT | 258bp | 58°C | E6 & E7 |
| R: CAGTAGAGATCAGTTGTCTCTGGTTGC | |||||
| J04353.1: HPV 31-124 | 3861 to 3962 | F: TTTGCTTTGCTTTTGTGTGCTAC | 124bp | 52°C | E5 |
| R: TGGAGAGGTTGCAATAACCCATA | |||||
| M12732.1: HPV 33-149 | 424 to 572 | F: TGTCAAAGACCTTTGTGTCCTC | 149bp | 54°C | E6 |
| R: GGCGTTTTTACACGTCACAG |