Table 3.

Epigenetically Linked Progressive Laryngeal Cases

RRP	Lesion Type	Biospy	Time Interval	BRCA2	APC	CDKN2A	CDKN2B	HPV (Roche)	HPV (PCR)
Case 1	Squamous Papilloma	1	Primary	M*	M†	U*	U*	16	16
	Squamous Papilloma with severe dysplasia	2	4 months	M†	M†	U*	U		16
	Primary SCC, Block 1 tumor	3T	6 months	M†	U*	U*	U		16 & 33
	Primary SCC, Block 1 dysplastic papilloma	3P	6months	M†	M†	U*	U		16
	Recurrent Severe Dysplasia	4	50 months	M†	M*	M†	U*	neg	neg
	Carcinoma in Situ	5	51 months	M†	M†	M*	U*	6	neg
	Recurrent SCC	6	53 months	M†	M†	M*	U, NR by MSP	not informative	neg
Case 5	Squamous Papilloma with moderate to severe dysplasia	1	Reference	M†	M*	M*	U*	neg	11
	Squamous Papilloma with mild to moderate dysplasia	2	2 months	M†	M†	M*	M	neg	neg
	Primary SCC	3	9 months	M†	M†	M*	M	neg	neg
	Carcinoma in Situ	4	11 months	M†	M*	M†	U*	neg	neg

RRP = Recurrent Respiratory Papilloma

SCC = Squamous Cell Carcinoma

M = methylation detected by MS-MLPA only

M^† = methylation detected by MSP only

M* = MS-MLPA methylation confirmed by MSP

U = unmethylated by MS-MLPA only

U* = unmethylated by MS-MLPA and MSP

NR = no reaction by MSP because of insufficient DNA

neg = negative for HPV