Figure 11.

Fractionation of Mos10 by density gradient centrifugation. A whole cell extract of strain RKY1452 (MOS10-13myc) was fractionated on a sucrose density gradient (20–50% wt/wt sucrose, fraction 1: low-sucrose density). (A) Gradient fractions were analyzed for the presence of marker proteins by Western blotting with specific antibodies as indicated. (B) Densitometric quantification of the Western blot signals. The Western blots were scanned and the signal intensities were quantified with the program NIH Image 1.62. The strongest signals were set to 100%. Pep12 (○), ALP (▵), and Mos10-[13myc (▪).