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. 2001 Mar;12(3):711–723. doi: 10.1091/mbc.12.3.711

Figure 5.

Figure 5

CPY sorting in deletion mutants. Cells were labeled for 10 min with [35S] Trans label and chased with an excess of cold methionine and cysteine for 40 min. CPY was immunoprecipitated from spheroplast (internal, I) fractions and medium (external, E) fractions. The precipitated proteins were analyzed by SDS-PAGE and autoradiography. Lanes 5 and 6, JD52 (wild-type); lanes 7 and 8, RKY1452 (MOS10-13myc); lanes 9 and 10, RKY1633 (VPS20-13myc); lanes 11 and 12, RKY1509 (Δmos10); lanes 13 and 14, RKY1590 (Δvps20); lanes 15 and 16, RKY1510 (Δsnf7); and lanes 17 and 18, RKY1511 (Δvps4). For comparison, CPY maturation was followed in the wild-type strain JD52 (lanes 1–4). Cells were briefly labeled (5 min) and chased for the time periods indicated. CPY was immunoprecipitated from whole cell extracts. The positions of the different CPY forms are indicated.

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