Figure 6.
CPY sorting control. Cells were labeled for 5 min with [35S] Trans label and chased with an excess of cold methionine and cysteine. CPY was immunoprecipitated from spheroplast (internal, I) fractions and medium (external, E) fractions at 0 and 40 min of chase. The precipitated proteins were analyzed by SDS-PAGE and autoradiography. Lanes 1–4, JD52 (wild-type); lanes 5–8, RKY1509 (Δmos10); and lanes 9–12, RKY1511 (Δvps4). The positions of the different CPY forms are indicated. The autoradiograms were scanned and the CPY signals were quantified with the program NIH Image 1.62.