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FM4-64 internalization in the deletion mutants. Cells were incubated for 30 min with the lipophilic dye FM4-64. After a 45–60-min chase period in medium without dye, FM4-64 fluorescence was examined. (A) JD52 (wild-type), (B) JD52 (10 mM NaN3 + 10 mM NaF added together with FM4-64), (C) RKY1511 (Δvps4), (D) RKY1509 (Δmos10), (E) RKY1590 (Δvps20), and (F) RKY1510 (Δsnf7). (Left) FM4-64 fluorescence. (Right) Nomarsky image.
