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Localization of Ste6 by immunofluorescence. The c-myc-tagged Ste6 variant, encoded by pYKS2 (Kuchler et al., 1993), was detected with anti-myc primary antibodies (9E10) and FITC-conjugated anti-mouse secondary antibodies in different strains. JD52 (wild-type) (A), RKY1511 (Δvps4) (B), RKY1509 (Δmos10) (C), RKY1590 (Δvps20) (D), and RKY1510 (Δsnf7) (E). (Left) FITC-fluorescence. (Right) Phase contrast image.
