Figure 9.

Fractionation of Mos10 and Vps20 by differential centrifugation. Cleared cell extracts were centrifuged at 13,000 × g for 10 min to pellet the P13 fraction. The supernatant was separated into a P100 pellet fraction and a S100 supernatant fraction by an additional centrifugation at 100,000 × g for 1 h. Equal portions of the fractions were analyzed by Western blotting with 9E10 antibodies directed against the 13myc-tagged proteins. RKY1452 (MOS10-13myc) (A), RKY1452 (MOS10-13myc + 2% Triton X-100) (B), RKY1633 (VPS20-13myc) (C), and RKY1633 (VPS20-13myc + 2% Triton X-100) (D). (Top) Western blots. (Bottom) Quantification of the Western blot signals. The signals of the total fractions were set to 100%. The average percentages (with SDs) of several experiments (n = 2–4) are shown.